# User guide

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     Hand edits here are overwritten by `pnpm autogen`; edit the script. -->

How to drive JBrowse once it is running. New here? Start with the
[JBrowse Web](https://jbrowse.org/jb2/docs/quickstart_web) or
[JBrowse Desktop](https://jbrowse.org/jb2/docs/quickstart_desktop) quickstart.

## General usage

- [Basic usage](https://jbrowse.org/jb2/docs/user_guides/basic_usage)
- [Connections](https://jbrowse.org/jb2/docs/user_guides/connections)
- [Editing a track's plot as text](https://jbrowse.org/jb2/docs/user_guides/edit_plot)
- [Export R script](https://jbrowse.org/jb2/docs/user_guides/r_export)
- [Highlights](https://jbrowse.org/jb2/docs/user_guides/highlights)
- [Installing plugins](https://jbrowse.org/jb2/docs/user_guides/plugin_store)
- [Opening track hubs by URL](https://jbrowse.org/jb2/docs/user_guides/hub_url)

## Track types

- [Alignments track](https://jbrowse.org/jb2/docs/user_guides/alignments_track)
- [GC content track](https://jbrowse.org/jb2/docs/user_guides/gc_content_track)
- [Gene track](https://jbrowse.org/jb2/docs/user_guides/gene_track)
- [GWAS / Manhattan track](https://jbrowse.org/jb2/docs/user_guides/gwas_track)
- [Hi-C track](https://jbrowse.org/jb2/docs/user_guides/hic_track)
- [MAF track](https://jbrowse.org/jb2/docs/user_guides/maf_track)
- [Multi-row feature track](https://jbrowse.org/jb2/docs/user_guides/multirow_feature_track)
- [Multi-sample variant display](https://jbrowse.org/jb2/docs/user_guides/multivariant_track)
- [Quantitative track](https://jbrowse.org/jb2/docs/user_guides/quantitative_track)
- [Sequence track](https://jbrowse.org/jb2/docs/user_guides/sequence_track)
- [Variant track](https://jbrowse.org/jb2/docs/user_guides/variant_track)

## Views

- [Circular genome view](https://jbrowse.org/jb2/docs/user_guides/circular_view)
- [Dotplot view](https://jbrowse.org/jb2/docs/user_guides/dotplot_view)
- [Graph genome view](https://jbrowse.org/jb2/docs/user_guides/graph_genome_view)
- [Linear synteny view](https://jbrowse.org/jb2/docs/user_guides/linear_synteny_view)
- [Spreadsheet view](https://jbrowse.org/jb2/docs/user_guides/spreadsheet_view)
- [SV inspector view](https://jbrowse.org/jb2/docs/user_guides/sv_inspector_view)

## Sequence tools

- [BLAT and in-silico PCR](https://jbrowse.org/jb2/docs/user_guides/blat)
- [Consensus sequence](https://jbrowse.org/jb2/docs/user_guides/consensus_sequence)
- [Feature sequence panel](https://jbrowse.org/jb2/docs/user_guides/feature_sequence)
- [Sequence search](https://jbrowse.org/jb2/docs/user_guides/sequence_search)

## Analysis

- [Clustering rows](https://jbrowse.org/jb2/docs/user_guides/clustering)
- [SV visualization](https://jbrowse.org/jb2/docs/user_guides/sv_visualization)

## Tutorials

### genomes.jbrowse.org

- [Basic usage of genomes.jbrowse.org](https://jbrowse.org/jb2/docs/tutorials/genomes_basics)
- [Synteny on genomes.jbrowse.org](https://jbrowse.org/jb2/docs/tutorials/genomes_synteny)
- [Proteins on genomes.jbrowse.org](https://jbrowse.org/jb2/docs/tutorials/genomes_proteins)
- [RepeatMasker as one row per class](https://jbrowse.org/jb2/docs/tutorials/repeatmasker_classes)

### Synteny & comparative genomics

#### Whole-genome alignments

- [Synteny visualization (pairwise minimap2)](https://jbrowse.org/jb2/docs/tutorials/synteny_visualization)
- [Comparing one genome's two haplotypes (T2T-HG002)](https://jbrowse.org/jb2/docs/tutorials/hg002_haplotypes)
- [Synteny from liftOver chains (hg38 and eight vertebrates)](https://jbrowse.org/jb2/docs/tutorials/hg38_vertebrates_synteny)
- [Synteny visualization (all-vs-all minimap2)](https://jbrowse.org/jb2/docs/tutorials/allvsall_synteny)
- [Rearrangements between genomes by type (SyRI)](https://jbrowse.org/jb2/docs/tutorials/syri_synteny)
- [Synteny on a circle (human and mouse)](https://jbrowse.org/jb2/docs/tutorials/circular_synteny)

#### Ortholog tables

- [Synteny from MCScan anchors (grape, peach)](https://jbrowse.org/jb2/docs/tutorials/mcscan_synteny_grape_peach)
- [Synteny from an ortholog table (grape, peach, cacao)](https://jbrowse.org/jb2/docs/tutorials/multiway_synteny_grape_peach_cacao)
- [Synteny visualization (OrthoFinder orthogroups)](https://jbrowse.org/jb2/docs/tutorials/orthofinder_synteny)
- [Synteny from gene symbols (eight primates)](https://jbrowse.org/jb2/docs/tutorials/primate_orthologs_synteny)
- [Synteny from gene symbols (44 E. coli genomes)](https://jbrowse.org/jb2/docs/tutorials/ecoli_orthologs_synteny)
- [Synteny by ancestral linkage group (sponge, comb jelly, jellyfish)](https://jbrowse.org/jb2/docs/tutorials/odp_linkage_groups_synteny)
- [Selection pressure between two genomes (dN/dS)](https://jbrowse.org/jb2/docs/tutorials/selection_pressure)
- [Synteny visualization (a polyploid against itself)](https://jbrowse.org/jb2/docs/tutorials/homoeolog_synteny)

### Pangenomes

- [Pangenome (pggb)](https://jbrowse.org/jb2/docs/tutorials/pangenome_ecoli)
- [Pangenome (Minigraph-Cactus)](https://jbrowse.org/jb2/docs/tutorials/pangenome_cactus)
- [Pangenome (hosting your own graph)](https://jbrowse.org/jb2/docs/tutorials/pangenome_prepare_graph)
- [Pangenome (mouse)](https://jbrowse.org/jb2/docs/tutorials/pangenome_mouse)
- [Pangenome (cattle)](https://jbrowse.org/jb2/docs/tutorials/pangenome_cattle)

#### HPRC release 2

- [](https://jbrowse.org/jb2/docs/tutorials/pangenome_hprc)
- [Pangenome (HPRC) part 2: haplotypes against each other](https://jbrowse.org/jb2/docs/tutorials/pangenome_hprc_haplotypes)
- [Pangenome (HPRC) part 3: repeat lengths across haplotypes](https://jbrowse.org/jb2/docs/tutorials/pangenome_hprc_repeats)

### Structural variation

- [CNV across a population (1000 Genomes)](https://jbrowse.org/jb2/docs/tutorials/population_cnv)
- [Structural variants (1000 Genomes)](https://jbrowse.org/jb2/docs/tutorials/sv_multisamples)
- [Structural variants from Hi-C](https://jbrowse.org/jb2/docs/tutorials/hic_structural_variants)
- [Low-mappability regions (SMN)](https://jbrowse.org/jb2/docs/tutorials/mappability_qc)

### Cancer genomics

- [Complex rearrangements and derivative alleles](https://jbrowse.org/jb2/docs/tutorials/cancer_sv)
- [Gene fusion calls and the DNA behind them](https://jbrowse.org/jb2/docs/tutorials/k562_fusions)
- [Reviewing a whole SV callset](https://jbrowse.org/jb2/docs/tutorials/sv_callset_review)
- [Structural variants (Cancer GIAB)](https://jbrowse.org/jb2/docs/tutorials/sv_visualization_cgiab)
- [Mutation cohort (TCGA)](https://jbrowse.org/jb2/docs/tutorials/tcga_cohort_mutations)
- [CNV cohort (TCGA)](https://jbrowse.org/jb2/docs/tutorials/tcga_cohort_cnv)

### Population genomics

- [Selection scans (Drosophila DGRP)](https://jbrowse.org/jb2/docs/tutorials/population_genomics)
- [Phased trio analysis (1000 Genomes)](https://jbrowse.org/jb2/docs/tutorials/analyze_trio)
- [LD at a selective sweep (human)](https://jbrowse.org/jb2/docs/tutorials/ld_human)
- [LD across an inversion (mosquitoes)](https://jbrowse.org/jb2/docs/tutorials/ld_mosquitoes)
- [QTL mapping (BXD mice)](https://jbrowse.org/jb2/docs/tutorials/bxd_qtl)

#### Dog10K

- [A selected haplotype (Dog10K)](https://jbrowse.org/jb2/docs/tutorials/dog10k_selection)
- [A loss-of-function allele across breeds (Dog10K)](https://jbrowse.org/jb2/docs/tutorials/dog10k_lof)
- [Structural variants (Dog10K)](https://jbrowse.org/jb2/docs/tutorials/dog10k_svs)
- [Local ancestry (Dog10K)](https://jbrowse.org/jb2/docs/tutorials/local_ancestry)

### Epigenomics & single cell

- [Methylation (long-read)](https://jbrowse.org/jb2/docs/tutorials/methylation)
- [Methylation (bisulfite)](https://jbrowse.org/jb2/docs/tutorials/bisulfite)
- [ChromHMM chromatin states](https://jbrowse.org/jb2/docs/tutorials/chromhmm)
- [Single-cell ATAC pseudobulk](https://jbrowse.org/jb2/docs/tutorials/scatac_pseudobulk)
- [Single-cell RNA pseudobulk](https://jbrowse.org/jb2/docs/tutorials/scrna_pseudobulk)
- [AlphaGenome predictions](https://jbrowse.org/jb2/docs/tutorials/alphagenome)

### Transcriptomics & proteins

- [RNA-seq visualization](https://jbrowse.org/jb2/docs/tutorials/rnaseq)
- [Differential transcript usage](https://jbrowse.org/jb2/docs/tutorials/dtu)
- [TP53 from prediction to crystal](https://jbrowse.org/jb2/docs/tutorials/tp53_structures)

### Genes & annotation

- [Reviewing a gene prediction (Tiberius)](https://jbrowse.org/jb2/docs/tutorials/gene_prediction_review)
- [Gene density and transposon density along a chromosome](https://jbrowse.org/jb2/docs/tutorials/gene_density)
- [A map of the human mitochondrial genome](https://jbrowse.org/jb2/docs/tutorials/organelle_map)

### Grammar of graphics

- [A grammar of graphics over a BED (RepeatMasker Alu age)](https://jbrowse.org/jb2/docs/tutorials/alu_age)
- [A grammar of graphics over a BAM (NA12878 insert size)](https://jbrowse.org/jb2/docs/tutorials/read_marks)

### Configuration & embedding

- [Display settings](https://jbrowse.org/jb2/docs/tutorials/display_settings)
- [Embedding JBrowse](https://jbrowse.org/jb2/docs/tutorials/embed_linear_genome_view)
- [JBrowse CLI with Desktop](https://jbrowse.org/jb2/docs/tutorials/cli_desktop)

### Automation

- [Driving JBrowse with an AI agent (two Drosophila genomes)](https://jbrowse.org/jb2/docs/tutorials/agent_synteny)

